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Reaktivering af demembranerede cellemodeller i Chlamydomonas reinhardtii
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Biologia
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JoVE Journal Biologia
Reactivation of Demembranated Cell Models in Chlamydomonas reinhardtii

Reaktivering af demembranerede cellemodeller i Chlamydomonas reinhardtii

DOI:
10.3791/63869-v

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03:37 min

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May 06, 2022

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, ,
1Science Research Center,Hosei University, 2Laboratory for Chemistry and Life Science, Institute of Innovative Research,Tokyo Institute of Technology, 3School of Life Science and Technology,Tokyo Institute of Technology

Capítulos

  • 00:04Introduction
  • 00:46Preparation of Demembranated Cell Models
  • 01:47Reactivation of Demembranated Cell Models
  • 02:16Results: Demembranation and Reactivation of Chlamydomonas reinhardtii Cell Models
  • 02:56Conclusion

Summary

Tadução automática

Tadução automática

In vitro-reaktivering af bevægelige celler er et afgørende eksperiment i forståelsen af mekanismerne for cellemotilitet. Protokollen beskriver genaktivering af de demembranerede cellemodeller af Chlamydomonas reinhardtii, en modelorganisme til undersøgelse af cilia/flagella.

Tags

Demembranated Cell ModelsChlamydomonas ReinhardtiiCilia MotilityIn Vitro ObservationCalcium Ion ConcentrationsMotile Cilia ResearchCentrifugationWashing BufferDemembranation BufferReactivation SolutionATP SolutionMicroscopyCell Models Reactivation

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