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1Department of Chemical Engineering,Imperial College London, 2Imperial College Centre for Synthetic Biology,Imperial College London, 3BioPharm Process Research,GlaxoSmithKline Research and Development, 4Department of Life Science,Imperial College London, 5School of Chemical & Bioprocess Engineering,University College Dublin
Capítulos
- 00:04Introduction
- 01:00DsiRNA Transfection
- 02:17Buffer Exchange and Sample Concentration
- 03:19Glycan Analysis
- 05:18Results: Confirmation of RNA Interference and Effect of Fut8 Knockdown
- 06:12Conclusion
The glycosylation pattern of an antibody determines its clinical performance, thus industrial and academic efforts to control glycosylation persist. Since typical glycoengineering campaigns are time- and labor-intensive, the generation of a rapid protocol to characterize the impact of glycosylation genes using transient silencing would prove useful.
