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1Marsico Lung Institute / CF Center,The University of North Carolina at Chapel Hill, 2Rheonova, 3Department of Pulmonary and Critical Care Medicine,The University of North Carolina at Chapel Hill, 4Department of Pediatric, Pediatric Pulmonology Division,The University of North Carolina at Chapel Hill
Capítulos
- 00:04Introduction
- 01:22Sputum or Mucus Collection and Storage
- 02:11Sample Preparation
- 03:17Instrument Initialization and Calibration
- 04:21Sample Loading
- 05:49Initiate Biophysical Measurement and Sample Removal
- 06:33Results: Using Benchtop Rheometer to Monitor Changes in Mucus Viscoelasticity
- 08:05Conclusion
Les propriétés viscoélastiques du mucus jouent un rôle essentiel dans la clairance mucociliaire. Cependant, les techniques rhéologiques traditionnelles du mucus nécessitent des approches complexes et chronophages. Cette étude fournit un protocole détaillé pour l’utilisation d’un rhéomètre de paillasse capable d’effectuer rapidement et de manière fiable des mesures viscoélastiques.
