Culturing and Genetically Manipulating Entomopathogenic Nematodes

Christa Heryanto; Ramesh Ratnappan; Damien M. O'Halloran; John M. Hawdon; Ioannis Eleftherianos

doi:10.3791/63885

Journal

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Imunologia e Infecção

/

培养和遗传操纵昆虫致病线虫

JoVE Journal
Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Culturing and Genetically Manipulating Entomopathogenic Nematodes

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培养和遗传操纵昆虫致病线虫

DOI:

10.3791/63885-v

•

07:17 min

•

March 31, 2022

•

Christa Heryanto, Ramesh Ratnappan, Damien M. O’Halloran, John M. Hawdon, Ioannis Eleftherianos

¹Department of Biological Sciences,The George Washington University, ²Department of Microbiology, Immunology, and Tropical Medicine, School of Medicine and Health Sciences,The George Washington University

Capítulos

00:04Introduction
00:36Production of Symbiotic Nematode Infective Juveniles
02:27Heterorhabditis bacteriophora Infective Juveniles and Surface Sterilizing
05:55Results: Validation of Heterorhabditis bacteriophora Axenization
06:24Conclusion

Summary

Tadução automática

昆虫致病线虫与细菌共生，它们通过破坏其先天免疫系统成功地感染昆虫。为了促进线虫感染的遗传基础研究，描述了维持和遗传操纵昆虫致病线虫的方法。

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