Journal
/
Imunologia e Infecção
/
Mycobacterium tuberculosis Extracellular Vesicle Enrichment through Size Exclusion Chromatography
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Mycobacterium tuberculosis Extracellular Vesicle Enrichment through Size Exclusion Chromatography

Mycobacterium tuberculosis Extracellular Vesicle Enrichment through Size Exclusion Chromatography

DOI:
10.3791/63895-v

•

00:00 min

•

May 19, 2022

•

, ,
1Mycobacteria Research Laboratories, Department of Microbiology Immunology & Pathology,Colorado State University, 2BioMARC, Infectious Disease Research Center,Colorado State University

Capítulos

  • 00:04Introduction
  • 00:50Preparation of Crude Mtb EV Concentrate
  • 02:36Size Exclusion Chromatography for the Enrichment of Mtb EVs from CFP
  • 05:52Quantification and Qualification of the Mtb EVs
  • 07:25Results: Silver Stain and Western Blots by Fraction, TEM Images, and NTA Distribution
  • 08:42Conclusion

Summary

Tadução automática

Tadução automática

This protocol describes size exclusion chromatography, a facile and reproducible technique for enriching Mycobacterium tuberculosis extracellular vesicles from culture supernatants.

Tags

Keywords: Mycobacterium TuberculosisExtracellular VesiclesSize Exclusion ChromatographyEnrichmentUltracentrifugationCentrifugal FilterPBSBCA AssaySize Exclusion Chromatography ColumnAFC Tower

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/63895?article_type=v

Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies
check_url/pt/63895?article_type=v

Growth of Mycobacterium tuberculosis Biofilms
check_url/pt/63895?article_type=v

An Experimental Model to Study Tuberculosis-Malaria Coinfection upon Natural Transmission of Mycobacterium tuberculosis and Plasmodium berghei
check_url/pt/63895?article_type=v

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
check_url/pt/63895?article_type=v

A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas
check_url/pt/63895?article_type=v

A 3D Human Lung Tissue Model for Functional Studies on Mycobacterium tuberculosis Infection
check_url/pt/63895?article_type=v

System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
check_url/pt/63895?article_type=v

A High-throughput Compatible Assay to Evaluate Drug Efficacy against Macrophage Passaged Mycobacterium tuberculosis
check_url/pt/63895?article_type=v

Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence
check_url/pt/63895?article_type=v

Evaluation of Extracellular Vesicle Function During Malaria Infection

Read Article