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将质粒DNA电穿孔到小鼠骨骼肌中

JoVE Journal
Biologia

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JoVE Journal Biologia

Electroporation of Plasmid DNA into Mouse Skeletal Muscle

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将质粒DNA电穿孔到小鼠骨骼肌中

DOI:

10.3791/63916-v

•

06:20 min

•

April 06, 2022

•

Brian A. Hain, David L. Waning

¹Department of Cellular and Molecular Physiology,Penn State College of Medicine

Capítulos

00:04Introduction
00:55Tibialis Anterior (TA) Muscle Injection/Electroporation
02:55EDL Injection/Electroporation
04:35Results: Transient Gene Expression Modulation in Murine Skeletal Muscle by Plasmid Electroporation
05:31Conclusion

Summary

Tadução automática

将质粒DNA电穿孔到骨骼肌中是一种在不损害小鼠肌肉收缩力的情况下调节基因表达的可行方法。

Tags

Keywords: Electroporation Plasmid DNA Mouse Skeletal Muscle Gene Transfer Muscle Contractility In Vivo Tibialis Anterior Needle Injection Caliper Electrodes Square Wave Pulses

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