Journal
/
Imunologia e Infecção
/
Quantitative 3D Imaging of Trypanosoma cruzi-Infected Cells, Dormant Amastigotes, and T Cells in Intact Clarified Organs
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Quantitative 3D Imaging of Trypanosoma cruzi-Infected Cells, Dormant Amastigotes, and T Cells in Intact Clarified Organs

Quantitative 3D Imaging of Trypanosoma cruzi-Infected Cells, Dormant Amastigotes, and T Cells in Intact Clarified Organs

DOI:
10.3791/63919-v

•

08:17 min

•

June 23, 2022

•

, , , ,
1Center for Tropical and Emerging Global Diseases,University of Georgia, 2Department of Cellular Biology,University of Georgia

Capítulos

  • 00:04Introduction
  • 01:10Tissue Clearing and DNA Staining
  • 02:40Immunostaining
  • 04:27RI Matching and Mounting
  • 05:22Results I: Visualization of Trypanosoma cruzi‐Infected Cells, Dormant Amastigotes, and Inflammation Foci in Cleared Organs
  • 06:27Results II: Immunodetection of Vasculature in Cleared Trypanosoma cruzi‐Infected Organs
  • 07:07Conclusion

Summary

Tadução automática

Tadução automática

The present protocol describes light-sheet fluorescent microscopy and automated software-assisted methods to visualize and precisely quantify proliferating and dormant Trypanosoma cruzi parasites and T cells in intact, cleared organs and tissues. These techniques provide a reliable way to evaluate treatment outcomes and offer new insights into parasite-host interactions.

Tags

AI3D ImagingTrypanosoma CruziAmastigotesT CellsChagas DiseaseCUBIC-LImmunostainingMicroscopy

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/63919?article_type=v

Retroviral Transduction of T-cell Receptors in Mouse T-cells
check_url/pt/63919?article_type=v

Ex vivo Imaging of T Cells in Murine Lymph Node Slices with Widefield and Confocal Microscopes
check_url/pt/63919?article_type=v

Following Cell-fate in E. coli After Infection by Phage Lambda
check_url/pt/63919?article_type=v

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
check_url/pt/63919?article_type=v

piggyBac Transposon System Modification of Primary Human T Cells
check_url/pt/63919?article_type=v

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells
check_url/pt/63919?article_type=v

Derivation of T Cells In Vitro from Mouse Embryonic Stem Cells
check_url/pt/63919?article_type=v

T Cells Capture Bacteria by Transinfection from Dendritic Cells
check_url/pt/63919?article_type=v

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data
check_url/pt/63919?article_type=v

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol

Read Article