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Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture
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JoVE Journal Bioengenharia
Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture

Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture

DOI:
10.3791/63935-v

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10:00 min

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July 20, 2022

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, , , , , , ,
1Department of Chemical and Biochemical Engineering,Rutgers University, 2Department of Biomedical Engineering,Rutgers University, 3Department of Medicine,Rutgers Biomedical Health Sciences

Capítulos

  • 00:04Introduction
  • 00:26Measure Component RTDs and Perform Signal Convolution
  • 02:51Set up the Perfusion System with a Stopcock for Multiple Medium Sources
  • 07:26Results: Assembly and Operation of a Perfusion Cell Culture System with Multiple Medium Sources
  • 09:32Conclusion

Summary

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This paper presents a method to construct and operate a low-cost, multichannel perfusion cell culture system for measuring the dynamics of secretion and absorption rates of solutes in cellular processes. The system can also expose cells to dynamic stimulus profiles.

Tags

Multi-stream Perfusion BioreactorOutlet FractionationDynamic Cell CultureSecretion RatesTime-varying Solid SignalsLow-costLittle TrainingPerfusion System SetupTracer SolutionBackground SolutionFour-way StopcockSyringe PumpsRTD AnalysisFlow SystemFraction CollectorSterile ConditionsWell Plate CulturesPerfusionIncubator

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