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多流灌注生物反应器集成了出口分馏功能，用于动态细胞培养

JoVE Journal
Bioengenharia

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JoVE Journal Bioengenharia

Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture

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多流灌注生物反应器集成了出口分馏功能，用于动态细胞培养

DOI:

10.3791/63935-v

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10:00 min

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July 20, 2022

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Patrick Erickson, Aneesha Doshi, Gunjan Jetley, Param Amin, Aamena Mejevdiwala, Ashna Patel, Raphaela Bento, Biju Parekkadan³

¹Department of Chemical and Biochemical Engineering,Rutgers University, ²Department of Biomedical Engineering,Rutgers University, ³Department of Medicine,Rutgers Biomedical Health Sciences

Capítulos

00:04Introduction
00:26Measure Component RTDs and Perform Signal Convolution
02:51Set up the Perfusion System with a Stopcock for Multiple Medium Sources
07:26Results: Assembly and Operation of a Perfusion Cell Culture System with Multiple Medium Sources
09:32Conclusion

Summary

Tadução automática

本文提出了一种构建和操作低成本、多通道灌注细胞培养系统的方法，用于测量细胞过程中溶质分泌和吸收速率的动态。该系统还可以使细胞暴露于动态刺激曲线中。

Tags

Keywords: Multi-stream Perfusion Bioreactor Outlet Fractionation Dynamic Cell Culture Secretion Rates Time-varying Solid Signals Low-cost Little Training Perfusion System Setup Tracer Solution Background Solution Four-way Stopcock Syringe Pumps RTD Analysis Flow System Fraction Collector Sterile Conditions Well Plate Cultures Perfusion Incubator

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