/
/
Multi-Stream Perfusion Bioreactor Integreret med Udløbsfraktionering til dynamisk cellekultur
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:04Introduction
- 00:26Measure Component RTDs and Perform Signal Convolution
- 02:51Set up the Perfusion System with a Stopcock for Multiple Medium Sources
- 07:26Results: Assembly and Operation of a Perfusion Cell Culture System with Multiple Medium Sources
- 09:32Conclusion
Dette papir præsenterer en metode til at konstruere og drive et billigt, multikanals perfusionscellekultursystem til måling af dynamikken i sekretion og absorptionshastigheder for opløste stoffer i cellulære processer. Systemet kan også udsætte celler for dynamiske stimulusprofiler.
Tags
Keywords:
Multi-stream Perfusion BioreactorOutlet FractionationDynamic Cell CultureSecretion RatesTime-varying Solid SignalsLow-costLittle TrainingPerfusion System SetupTracer SolutionBackground SolutionFour-way StopcockSyringe PumpsRTD AnalysisFlow SystemFraction CollectorSterile ConditionsWell Plate CulturesPerfusionIncubator
