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Self-Assembly of Microtubule Tactoids
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Self-Assembly of Microtubule Tactoids

Self-Assembly of Microtubule Tactoids

DOI:
10.3791/63952-v

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08:49 min

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June 23, 2022

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, , , , , , ,
1Physics Department,Syracuse University, 2Department of Molecular Biophysics and Biochemistry,Yale School of Medicine

Capítulos

  • 00:04Introduction
  • 00:42Tubulin Preparation
  • 02:10Assembly of Flow Chambers
  • 03:14Tactoid Experiments
  • 04:58Fluorescence Microscopy
  • 06:54Results: Formation of Microtubule Tactoids
  • 08:11Conclusion

Summary

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Tadução automática

This article presents a protocol for the formation of microtubule assemblies in the shape of tactoids using MAP65, a plant-based microtubule crosslinker, and PEG as a crowding agent.

Tags

Keywords: MicrotubuleSelf-assemblyTactoidsMAP65TubulinRhodamine-labeledFlow ChamberLiquid CrystalSpindle-like Shapes

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