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慢病毒介导的shRNA递送到hESC和NPC，使用低成本阳离子聚合物聚乙亚胺（PEI）
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Biologia
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JoVE Journal Biologia
Lentiviral Mediated Delivery of shRNAs to hESCs and NPCs Using Low-cost Cationic Polymer Polyethylenimine (PEI)
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慢病毒介导的shRNA递送到hESC和NPC，使用低成本阳离子聚合物聚乙亚胺（PEI）

DOI:
10.3791/63953-v

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06:49 min

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May 24, 2022

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1Department of Biochemistry and Molecular Biology, College of Medicine and Health Sciences,United Arab Emirates University, 2Zayed Center for Health Sciences,United Arab Emirates University

Capítulos

  • 00:04Introduction
  • 00:28Transfection of HEK293T Cells Using Either Polymer Polyethylenimine (PEI) or Lipofectamine 3000 Reagent
  • 02:48Lentiviral Vectors (LVS) Collection and Ultracentrifugation
  • 05:07Results: Lentiviral Mediated Delivery of shRNAs in hESCs and NPCs Using Cationic Polymer PEI
  • 06:19Conclusion

Summary

Tadução automática

Tadução automática

利用低成本的阳离子聚合物聚乙氨基亚胺（PEI），我们生产了慢病毒颗粒，用于H9人胚胎干细胞（hESCs）中shRNA的稳定表达，并高效瞬时转导H9衍生的神经祖细胞（NPC）。

Tags

LentiviralShRNAHEK293TTransfectionPolyethyleniminePEICationic PolymerLow Protein Binding FilterSucrose CushionUltracentrifugationDMEMFBSPenicillin StreptomycinConfluencyPlasmid DNAPackagingLipofectamineVirus-containing MediaCentrifugation

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