É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:04Introduction
- 00:40Perfusion
- 01:34Regional Anatomy and Cryosectioning of the Muscle
- 02:55Multiple Fluorescent Staining with Neurofilament 200 (NF200), Vesicular Acetylcholine Transporter (VAChT), Alpha‐Bungarotoxin (α‐BTX), and Phalloidin (Pha)
- 04:55Observation and Analyses
- 06:40Results: Investigating Spatial Correlation of Neuromuscular Junction in Rat Medial Gastrocnemius Muscle
- 08:13Conclusion
Tags
Keywords:
Neuromuscular JunctionMedial Gastrocnemius MuscleRatMorphological CharacteristicsSchwann CellsPre-synapticPost-synapticImmunofluorescencePerfusionCryoprotectionCryosectioningTriton X-100Normal Donkey SerumNeurofilament 200Vesicular Acetylcholine TransporterDonkey Anti-rabbit AF 488Donkey Anti-goat AF 594
