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基于同步成像和流式细胞术检测治疗诱导的衰老癌细胞中的多种荧光衰老标志物

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Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells

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基于同步成像和流式细胞术检测治疗诱导的衰老癌细胞中的多种荧光衰老标志物

DOI:

10.3791/63973-v

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08:56 min

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July 12, 2022

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Eva Dovjak, Mario Mairhofer, Claudia Wöß, Jingjing Qi, Dorothy N. Y. Fan, Clemens A. Schmitt^2,3, Yong Yu

¹Johannes Kepler University Linz, ²Charité – University Medicine Berlin, ³Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association

Capítulos

00:05Introduction
00:55Stain DLBCL Cells with Different Senescence Markers
04:01Imaging Senescence Markers Using the Imaging Flow Cytometer System
07:12Results: Visualization and Quantification of Senescent Cells on a Single-Cell Level
08:01Conclusion

Summary

Tadução automática

在这里，我们提出了一种基于流式细胞术的方法，用于可视化和定量单个细胞中多种衰老相关标记物。

Tags

Keywords: Simultaneous Imaging Flow Cytometry Fluorescent Senescence Markers Therapy-induced Senescent Cancer Cells DLBCL Cell Lines EdU Chloroquine C12 FDG 2-phenylethyl-beta-d-thiogalactoside Paraformaldehyde Fixation Saponin Permeabilization

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