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使用原子力显微镜结合偏振显微镜测量肝硬度

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Measurement of Liver Stiffness Using Atomic Force Microscopy Coupled with Polarization Microscopy

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使用原子力显微镜结合偏振显微镜测量肝硬度

DOI:

10.3791/63974-v

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10:10 min

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July 20, 2022

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Srikant Ojha, Jan Pribyl, Simon Klimovic³, Daniel Hadraba, Marketa Jirouskova, Martin Gregor

¹Laboratory of Integrative Biology,Institute of Molecular Genetics of the Czech Academy of Sciences, ²CEITEC MU,Masaryk University, ³Department of Biochemistry, Faculty of Science,Masaryk University, ⁴Laboratory of Biomathematics,Czech-BioImaging IPHYS, Institute of Physiology of the Czech Academy of Sciences

Capítulos

00:04Introduction
00:52Sample Preparation II
01:47Measurement
05:32Data Analysis
07:52Results: Measurement of the Elastic Moduli of Collagen‐Rich Areas
09:19Conclusion

Summary

Tadução automática

我们提出了一种使用原子力显微镜测量正常和患病肝脏中富含胶原蛋白的区域的弹性模量的方案。同时使用偏振显微镜为定位肝脏切片中富含胶原蛋白的区域提供了高空间精度。

Tags

Liver Stiffness Atomic Force Microscopy Polarization Microscopy Collagen Fibrogenesis Liver Fibrosis Paraformaldehyde PBS AFM Cantilever Optical Calibration

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