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1Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases,The Third Affiliated Hospital of Guangzhou Medical University, 2Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, 3School of Biotechnology and Health Sciences,Wuyi University
Capítulos
- 00:04Introduction
- 00:51Induced Pluripotent Stem Cells (iPSCs) and EB Preparation (Days 0 1)
- 02:54Soft Light Lamp Preparation (Day 1), EB Transfer, and Medium Replacement (Days 2 5)
- 04:24Neural Induction (Days 5 7)
- 05:25Embedding in the Basement Membrane Matrix (Days 7 10)
- 06:52Organoid Maturation (Days 10 40)
- 07:21Results: Analysis of Cerebral Organoids
- 08:41Conclusion
Cerebrala organoider ger oöverträffade möjligheter att studera organutveckling och mänsklig sjukdomspatologi. Även om stor framgång har uppnåtts med cerebrala organoidkultursystem, finns det fortfarande operativa svårigheter att tillämpa denna teknik. Detta protokoll beskriver ett cerebralt organoidförfarande som underlättar mediumförändring och organoidöverföring.
