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Mikrotransplantation af synaptiske membraner til genaktivering af humane synaptiske receptorer til funktionelle undersøgelser
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Neurociência
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JoVE Journal Neurociência
Microtransplantation of Synaptic Membranes to Reactivate Human Synaptic Receptors for Functional Studies

Mikrotransplantation af synaptiske membraner til genaktivering af humane synaptiske receptorer til funktionelle undersøgelser

DOI:
10.3791/64024-v

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10:08 min

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July 20, 2022

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, , ,
1The Mitchell Center for Neurodegenerative Diseases, Department of Neurology,University of Texas Medical Branch

Capítulos

  • 00:04Introduction
  • 01:13Loading the Sample
  • 02:06Injecting the Oocytes
  • 02:49Recording of Ion Currents Using a Two-Electrode Voltage Clamp
  • 06:50Analyzing TEVC Recordings
  • 07:30Results: Representative Ion Currents of Oocytes Microtransplanted with Human Synaptic Receptors and Polarized Insertion of Receptors
  • 09:25Conclusion

Summary

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Protokollen viser, at ved at udføre mikrotransplantation af synaptiske membraner i Xenopus laevis oocytter er det muligt at registrere konsistente og pålidelige reaktioner af α-amino-3-hydroxy-5-methyl-4-isoxazolpropionsyre og γ-aminosmørsyrereceptorer.

Tags

MicrotransplantationSynaptic MembranesSynaptic ReceptorsFunctional StudiesNeurodegenerative DisordersMental Health DisordersSynaptic CommunicationSynaptic ReceptorsBrain ReceptorsReceptor FunctionalityReceptor ChangesReceptor ActivityInjectionNanoinjectorOocytesSample PreparationSonicationThermoplasticManipulatorFoot PedalPetri DishControl Group

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