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Microtransplantatie van synaptische membranen om menselijke synaptische receptoren voor functionele studies te reactiveren
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Capítulos
- 00:04Introduction
- 01:13Loading the Sample
- 02:06Injecting the Oocytes
- 02:49Recording of Ion Currents Using a Two-Electrode Voltage Clamp
- 06:50Analyzing TEVC Recordings
- 07:30Results: Representative Ion Currents of Oocytes Microtransplanted with Human Synaptic Receptors and Polarized Insertion of Receptors
- 09:25Conclusion
Het protocol toont aan dat door microtransplantatie van synaptische membranen in Xenopus laevis-eicellen uit te voeren, het mogelijk is om consistente en betrouwbare reacties van α-amino-3-hydroxy-5-methyl-4-isoxazolpropionzuur en γ-aminoboterzuurreceptoren vast te leggen.
Tags
MicrotransplantationSynaptic MembranesSynaptic ReceptorsFunctional StudiesNeurodegenerative DisordersMental Health DisordersSynaptic CommunicationSynaptic ReceptorsBrain ReceptorsReceptor FunctionalityReceptor ChangesReceptor ActivityInjectionNanoinjectorOocytesSample PreparationSonicationThermoplasticManipulatorFoot PedalPetri DishControl Group
