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In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles
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Bioengenharia
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JoVE Journal Bioengenharia
In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles

In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles

DOI:
10.3791/64026-v

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10:19 min

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August 25, 2022

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, ,
1Department of Biomedical Engineering,Yale University, 2Systems Biology Institute,Yale University, 3Department of Physics,Yale University

Capítulos

  • 00:04Introduction
  • 00:50Preparation of Buffers and Proteins Solutions
  • 03:07Preparation of Liposomes Based on the Inverted Emulsion Techniques
  • 05:51Microscopy Observation
  • 08:11Results: Microscopic Images of the Monolayer Lipid Droplet and Encapsulation of Actin Networks Inside Liposomes
  • 09:27Conclusion

Summary

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In this manuscript, we demonstrate the experimental techniques to encapsulate the F-actin cytoskeleton into giant unilamellar lipid vesicles (also called liposomes), and the method to form a cortex-biomimicking F-actin layer at the inner leaflet of the liposome membrane.

Tags

Actin CytoskeletonIn Vitro ReconstitutionGiant Unilamellar VesiclesLiposome YieldEncapsulation EfficiencyProtein EncapsulationMicroparticle EncapsulationSelf-propelling MicroswimmersNon-polymerization BufferPolymerization BufferEGGPCNickel Lipid

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