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Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells
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Bioengenharia
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JoVE Journal Bioengenharia
Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells

Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells

DOI:
10.3791/64036-v

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11:16 min

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September 09, 2022

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, , ,
1Joint Department of Biomedical Engineering,University of North Carolina at Chapel Hill and North Carolina State University, 2Comparative Medicine Institute,North Carolina State University, 3Lineberger Comprehensive Cancer Center,University of North Carolina at Chapel Hill

Capítulos

  • 00:10Introduction
  • 00:50Making the Macroporous Alginate Scaffolds
  • 05:47Transduction
  • 10:07Resultados
  • 10:51Conclusions

Summary

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Herein is a protocol for creating dry macroporous alginate scaffolds that mediate efficient viral gene transfer for use in genetic engineering of T cells, including T cells for CAR-T cell therapy. The scaffolds were shown to transduce activated primary T cells with >85% transduction.

Tags

Dry Macroporous Alginate ScaffoldsDryduxViral TransductionT CellsSpinoculationRetroNectinCalcium-D-gluconateAlginate SolutionLyophilization48-well Plate24-well Plate

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