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测量 秀丽隐杆线虫 对乙酰胆碱受体激动剂左旋咪唑的敏感性
JoVE Journal
Genética
This content is Free Access.
JoVE Journal Genética
Measuring Caenorhabditis elegans Sensitivity to the Acetylcholine Receptor Agonist Levamisole
DOI:

08:11 min

June 07, 2022

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Capítulos

  • 00:05Introduction
  • 00:45Preparation of Plates for the Levamisole Assay
  • 02:03Synchronizing C. elegans
  • 03:42Plating Synchronized C. elegans
  • 04:36Performing the Levamisole Assay
  • 05:47Data Analysis
  • 06:52Results: Levamisole-Induced Paralysis in Wild Type and Representative Mutant C. elegans
  • 07:28Conclusion

Summary

Tadução automática

本方案描述了一种测定以确定对左旋咪唑的反应,左旋咪唑是一类 秀丽隐杆线虫乙酰 胆碱受体的药理激动剂。在这种液体左旋咪唑游泳测定中,研究人员目视观察和定量在24孔板中培养的动物的时间依赖性麻痹。

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