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Biologia do Desenvolvimento
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Real Time and Repeated Measurement of Skeletal Muscle Growth in Individual Live Zebrafish Subjected to Altered Electrical Activity
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Real Time and Repeated Measurement of Skeletal Muscle Growth in Individual Live Zebrafish Subjected to Altered Electrical Activity

Real Time and Repeated Measurement of Skeletal Muscle Growth in Individual Live Zebrafish Subjected to Altered Electrical Activity

DOI:
10.3791/64063-v

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11:41 min

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June 16, 2022

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, , ,
1Randall Centre for Cell and Molecular Biophysics, School of Basic and Medical Biosciences,King’s College London

Capítulos

  • 00:05Introduction
  • 00:53Mounting Fish for Confocal Scanning
  • 03:05Confocal Scanning
  • 05:41Optional Method: Remove and Re-Introduce Muscle Electrical Activity
  • 09:01Results: Accurate and Efficient Estimation of the Muscle Volume of Live Zebrafish Larvae at Selected Stages of Activity
  • 11:01Conclusion

Summary

Tadução automática

Tadução automática

Optical clarity is a major advantage for cell biological and physiological work in zebrafish. Robust methods for measurement of cell growth in individual animals are described that permit novel insights into how growth of skeletal muscle and neighboring tissues are integrated with whole body growth.

Tags

Keywords: Skeletal Muscle GrowthLive ZebrafishAltered Electrical ActivityIn Vivo MeasurementReal-time MonitoringConfocal MicroscopyLow-melting AgaroseBiologia do DesenvolvimentoMuscle Wasting Disorders

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