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Endothelial Cell Transcytosis Assay as an In Vitro Model to Evaluate Inner Blood-Retinal Barrier Permeability
JoVE Journal
Medicina
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JoVE Journal Medicina
Endothelial Cell Transcytosis Assay as an In Vitro Model to Evaluate Inner Blood-Retinal Barrier Permeability
DOI:

10:56 min

June 07, 2022

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Capítulos

  • 00:04Introduction
  • 01:04Culturing HRMECs
  • 03:46TEER Measurements
  • 04:44Clathrin-Mediated Transcytosis Assay Using Cy3-Transferrin
  • 06:00Caveolae-Mediated Transcytosis Assay Using Horse Radish Peroxidase (HRP)
  • 07:29Results: Visualization of Endothelial Cell Transcytosis in the Retina and In Vitro Transcytosis Assay as a Model of BRB
  • 10:14Conclusion

Summary

Tadução automática

This protocol illustrates an in vitro endothelial cell transcytosis assay as a model to evaluate inner blood-retinal barrier permeability by measuring the ability of human retinal microvascular endothelial cells to transport horseradish peroxidase across cells in caveolae-mediated transcellular transport processes.

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