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内皮细胞转吞测定作为 体外 模型评估内部血-视网膜屏障通透性
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JoVE Journal Medicina
Endothelial Cell Transcytosis Assay as an In Vitro Model to Evaluate Inner Blood-Retinal Barrier Permeability
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内皮细胞转吞测定作为 体外 模型评估内部血-视网膜屏障通透性

DOI:
10.3791/64076-v

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10:56 min

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June 07, 2022

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, , , , , ,
1Department of Ophthalmology, Boston Children’s Hospital,Harvard Medical School

Capítulos

  • 00:04Introduction
  • 01:04Culturing HRMECs
  • 03:46TEER Measurements
  • 04:44Clathrin-Mediated Transcytosis Assay Using Cy3-Transferrin
  • 06:00Caveolae-Mediated Transcytosis Assay Using Horse Radish Peroxidase (HRP)
  • 07:29Results: Visualization of Endothelial Cell Transcytosis in the Retina and In Vitro Transcytosis Assay as a Model of BRB
  • 10:14Conclusion

Summary

Tadução automática

Tadução automática

该协议说明了 体外 内皮细胞转吞测定作为评估内部血 - 视网膜屏障通透性的模型，通过测量人视网膜微血管内皮细胞在洞穴介导的跨细胞运输过程中跨细胞运输辣根过氧化物酶的能力。

Tags

Endothelial Cell Transcytosis AssayIn Vitro ModelBlood-retinal Barrier PermeabilityVascular BiologyEye ResearchBrain ResearchBlood-brain BarrierTEER MeasurementCell CultureHuman Retinal Microvascular Endothelial CellsHRMECsCell SeedingCell Culture Filter InsertsGelatin Coating

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