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Capítulos
- 00:04Introduction
- 01:04Culturing HRMECs
- 03:46TEER Measurements
- 04:44Clathrin-Mediated Transcytosis Assay Using Cy3-Transferrin
- 06:00Caveolae-Mediated Transcytosis Assay Using Horse Radish Peroxidase (HRP)
- 07:29Results: Visualization of Endothelial Cell Transcytosis in the Retina and In Vitro Transcytosis Assay as a Model of BRB
- 10:14Conclusion
Detta protokoll illustrerar en in vitro-endotelcellstranscytosanalys som en modell för att utvärdera inre blod-retinal barriärpermeabilitet genom att mäta förmågan hos humana retinala mikrovaskulära endotelceller att transportera pepparrotsperoxidas över celler i caveolae-medierade transcellulära transportprocesser.
