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使用造血细胞因子接种肿瘤的实验性黑色素瘤免疫治疗模型

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Experimental Melanoma Immunotherapy Model Using Tumor Vaccination with a Hematopoietic Cytokine

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使用造血细胞因子接种肿瘤的实验性黑色素瘤免疫治疗模型

DOI:

10.3791/64082-v

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09:15 min

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February 24, 2023

•

Hsin Yu Liu, Amnon Altman, Ann J. Canonigo-Balancio, Michael Croft

¹La Jolla Institute for Immunology

Capítulos

00:04Introduction
00:56Preparation of Cultured Tumor Cells for Implantation
02:33Tumor Implantation
03:19Vaccine Preparation and Injection of Flt3L-Expressing B16 F10 (B16-Flt3) Cells
04:20Intratumoral Immune Cell Isolation
06:59Results: Using a Cell-Based Tumor Vaccine Expressing Flt3 Model to Study Intratumoral Immune Cell Infiltrates
08:45Conclusion

Summary

Tadução automática

该协议提出了一种癌症免疫治疗模型，使用表达Flt3L的B16-F10黑色素瘤进行基于细胞的肿瘤疫苗接种。该协议演示了程序，包括培养的肿瘤细胞的制备，肿瘤植入，细胞照射，肿瘤生长的测量，肿瘤内免疫细胞的分离和流式细胞术分析。

Tags

Keywords: Melanoma Immunotherapy Tumor Vaccination Hematopoietic Cytokine B16-F10 Cells Tumor Implantation Tumor Measurement Gamma Irradiation Cell Viability

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