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Levende billeddannelse af mikrotubulusdynamik i glioblastomceller, der invaderer zebrafiskhjernen
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Biologia
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JoVE Journal Biologia
Live Imaging of Microtubule Dynamics in Glioblastoma Cells Invading the Zebrafish Brain

Levende billeddannelse af mikrotubulusdynamik i glioblastomceller, der invaderer zebrafiskhjernen

DOI:
10.3791/64093-v

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09:29 min

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July 29, 2022

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, ,
1Cell Polarity, Migration and Cancer Unit,Université Paris-Cité, Institut Pasteur, Équipe Labellisée Ligue Contre le Cancer

Capítulos

  • 00:04Introduction
  • 01:03Xenotransplantation Procedure
  • 04:46Intravital Imaging of the Glioblastoma Xenografts
  • 07:41Results: Understanding Microtubule Dynamics of Glioblastoma Cells via Live Imaging of Zebrafish Larval Xenografts
  • 08:53Conclusion

Summary

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Vi rapporterer en teknik, der tillader levende billeddannelse af mikrotubulusdynamik i glioblastom (GBM) celler, der invaderer et hvirveldyrs hjernevæv. Kobling af den ortopiske injektion af fluorescerende mærkede GBM-celler til en gennemsigtig zebrafiskhjerne med intravital billeddannelse med høj opløsning muliggør måling af cytoskeletdynamik under in situ kræftinvasion.

Tags

Keywords: Microtubule DynamicsGlioblastoma CellsZebrafish BrainSubcellular Intravital ImagingBrain Cancer InvasionProtein AnalysisCell TransplantationMicroinjectionAnesthetized Larvae

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