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Journal / Developmental Biology / Fixation of Embryonic Mouse Tissue for Cytoneme…
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Developmental Biology

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JoVE Journal Developmental Biology
Fixation of Embryonic Mouse Tissue for Cytoneme Analysis
 

Fixation of Embryonic Mouse Tissue for Cytoneme Analysis

Article DOI: 10.3791/64100-v • 08:46 min • June 16th, 2022
June 16th, 2022 •
1, 1,2, 1, 1, 1
1Department of Cell and Molecular Biology, St. Jude Children’s Research Hospital, 2St. Jude Graduate School of Biomedical Sciences

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Here, an optimized step-by-step protocol is provided for fixation, immunostaining, and sectioning of embryos to detect specialized signaling filopodia called cytonemes in developing mouse tissues.

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Keywords: Embryonic Mouse Tissue Cytoneme Analysis Fixation Light Microscopy Endogenous Signaling Proteins Peritoneal Cavity Uterus Yolk Sac Placenta Paraformaldehyde HBSS PBS Triton Blocking Solution Primary Antibody Secondary Antibody Low Melting Point Agarose
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