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伯氏疏螺旋体（Borrelia）伯氏疏螺旋体外转录测定的基本成分

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JoVE Journal Imunologia e Infecção

Essential Components of Borreliella (Borrelia) burgdorferi In Vitro Transcription Assays

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伯氏疏螺旋体（Borrelia）伯氏疏螺旋体外转录测定的基本成分

DOI:

10.3791/64134-v

•

07:15 min

•

July 22, 2022

•

William K. Boyle, Hannah N. Sorensen, Travis J. Bourret

¹School of Medicine,Creighton University

Capítulos

00:04Introduction
00:50Purification of the RNA Polymerase and Preparation of RNA Polymerase Stock
03:35In Vitro Transcription with the Incorporation of Radiolabeled Nucleotides
05:35Results: Analysis of RpoD Purification and In Vitro Transcription
06:43Conclusion

Summary

Tadução automática

体外转录测定可以破译伯氏疏螺旋体的转录调控机制。该协议描述了纯化伯氏疏螺旋体RNA聚合酶并进行体外转录反应的步骤。使用体外转录测定的实验方法需要对活性RNA聚合酶进行可靠的纯化和储存。

Tags

Keywords: Borreliella Burgdorferi In Vitro Transcription Assay RNA Polymerase Gene Regulation Transcription Factors Drug Screening Cell Lysis Affinity Chromatography Cobalt Column Nickel Resin Column

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