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1College of Life Science,Northwest University, 2State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology,Fourth Military Medical University, 3Xi’an Institute of Tissue Engineering and Regenerative Medicine, 4School of Basic Medicine,Fourth Military Medical University, 5Department of Orthodontics, School of Stomatology,Fourth Military Medical University
Capítulos
- 00:05Introduction
- 01:04Differential Centrifugation for Isolation of Exosomes and Microvesicles
- 02:25Detection of Particle Numbers and Size Distribution of Extracellular Vesicles
- 03:29Labeling of Extracellular Vesicles Derived from Mesenchymal Stem Cells
- 04:50Systemic Injection of Extracellular Vesicles Derived from Mesenchymal Stem Cells
- 05:24Results: Characterization of Extracellular Vesicles Derived from Mesenchymal Stem Cells
- 06:19Conclusion
Das vorliegende Protokoll beschreibt die differentielle Zentrifugation zur Isolierung und Charakterisierung repräsentativer EVs (Exosomen und Mikrovesikel) aus kultivierten humanen MSCs. Weitere Anwendungen dieser Elektrofahrzeuge werden ebenfalls in diesem Artikel erläutert.
