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Embryo Injection Technique for Gene Editing in the Black-Legged Tick, Ixodes scapularis
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Biologia
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JoVE Journal Biologia
Embryo Injection Technique for Gene Editing in the Black-Legged Tick, Ixodes scapularis

Embryo Injection Technique for Gene Editing in the Black-Legged Tick, Ixodes scapularis

DOI:
10.3791/64142-v

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06:56 min

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September 13, 2022

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, , , ,
1Department of Biochemistry and Molecular Biology,University of Nevada, Reno, 2Insect Transformation Facility,University of Maryland Institute for Bioscience and Biotechnology Research, 3Department of Agriculture, Veterinary, and Rangeland Sciences,University of Nevada, Reno

Capítulos

  • 00:04Introduction
  • 01:29Preparation of Female Ticks for Embryo Collection
  • 02:34Embryo Treatment for Microinjections
  • 03:34Embryo Microinjections
  • 04:44Post-Injection Care of Embryos
  • 05:34Results: Embryo Injection in the Black-Legged Tick, Ixodes scapularis
  • 06:00Conclusion

Summary

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The present protocol describes a method for injecting tick embryos. Embryo injection is the preferred technique for genetic manipulation to generate transgenic lines.

Tags

Keywords: Embryo InjectionGene EditingBlack-legged TickIxodes ScapularisChelicerataTick Gene FunctionTick Pathogen InteractionsGene Drive SystemsLyme DiseaseImmune ResponseDNA RepairTick BiologyEgg LayingGland AblationBenzalkonium Chloride

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