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Farelerde gen kaseti knock-in ve flox alelleri oluşturmak için zigot mikroenjeksiyonu
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Capítulos
- 00:04Introduction
- 01:20Obtaining Mice Zygotes by Natural Mating
- 02:29Preparation of the Microinjection Needle and Zygote Microinjection
- 06:46Embryo Transfer into the Oviduct
- 07:15Results: Analysis of Production Efficiency of Microinjection
- 08:16Conclusion
Tags
Keywords:
Zygote MicroinjectionGene Cassette Knock-inFlox AllelesGenetically-modified MiceIn Vivo ExperimentsGene ExpressionGenetic HumanizationConditional Gene KnockoutNeurologyImmunologyMetabolismReproductionInfectious DiseaseM2 MediumHyaluronidaseM16 MediumMicroinjection NeedleMicroforgeMicromanipulatorPolyvinyl Pyrrolidone
