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Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
DOI:

09:32 min

October 14, 2022

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Capítulos

  • 00:05Introduction
  • 00:53Formaldehyde Fixation
  • 07:54Results: Capture Hi-C, 5C, and Hi-C Interaction Maps across the 3 Mb and 1 Mb Captured Region
  • 09:05Conclusion

Summary

Tadução automática

This protocol describes the Capture Hi-C method used to characterize the 3D organization of megabased-sized targeted genomic regions at high-resolution, including boundaries of topologically associating domains (TADs) and long-range chromatin interactions between regulatory and other DNA sequence elements.

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