É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
1Department of Physiology and Pathophysiology, Peking University School of Basic Medical Sciences, Key Laboratory of Molecular Cardiovascular Sciences,Ministry of Education, and Beijing Key Laboratory of Cardiovascular Receptors Research, 2Department of Oral and Maxillofacial Surgery,Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Reseah Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, 3State Key Laboratory of Natural and Biomimetic Drugs,Peking University
Capítulos
- 00:05Introduction
- 00:56Animal Procedures
- 02:43Two-Photon Microscope Set-up
- 03:17Vessel Imaging and Permeability Detection
- 05:01Results: Detecting Endothelial Barrier Function in the Mouse Submandibular Gland
- 06:30Conclusion
En el presente protocolo, la función de barrera endotelial de la glándula submandibular (SMG) se evaluó inyectando diferentes trazadores fluorescentes ponderados molecularmente en las venas angulares de modelos animales de prueba in vivo bajo un microscopio de escaneo láser de dos fotones.
