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使用蛋白质印迹法研究神经元K-Cl共转运蛋白KCC2的功能和活性
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting
DOI:

10:08 min

December 09, 2022

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Capítulos

  • 00:05Introduction
  • 00:50Cell Culture and Transfection
  • 02:28Preparation of Cell Lysates and Loading Samples
  • 03:11Preparation of Immunoprecipitants from Cell Lysates
  • 04:40Performing the Western Blot
  • 06:23Antibody Staining, Image Development, Image Acquisition, and Data Quantification
  • 07:16Results: Western Blotting Technique to Study the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2
  • 09:25Conclusion

Summary

Tadução automática

本协议重点介绍了蛋白质印迹技术在研究神经元K-Cl共转运蛋白KCC2的功能和活性方面的应用。该方案描述了通过蛋白质印迹 激酶调节位点Thr906/1007处KCC2磷酸化的研究。此外,本文还简要强调了确认KCC2活性的其他方法。

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