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Ontleedende cel-autonome functie van fragiele X mentale retardatie-eiwit in een auditief circuit door In Ovo-elektroporatie
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1Division of Histology & Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, College of Medicine,Jinan University, 2Department of Clinical Pathology,First Affiliated Hospital of Jinan University, 3Program in Neuroscience, Department of Biomedical Sciences, College of Medicine,Florida State University
Capítulos
- 00:05Introduction
- 00:50Egg Preparation
- 02:30Egg Windowing and Embryo Identification
- 03:39Neural Tube Injection and Electroporation
- 06:20Otocyst Injection and Electroporation
- 07:44Results: FMRP Knockdown in the Chicken NM and Auditory Duct and Axonal Tracking of Transfected AG Neurons in the Brainstem
- 10:18Conclusion
Met behulp van ovo-elektroporatie hebben we een methode ontwikkeld om selectief het auditieve binnenoor en de cochleaire kern in kippenembryo's te transfecteren om een celgroepspecifieke knockdown van fragiele X mentale retardatie-eiwit te bereiken tijdens discrete perioden van circuitassemblage.
