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微流体辅助轴突线粒体选择性去极化

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria

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微流体辅助轴突线粒体选择性去极化

DOI:

10.3791/64196-v

•

06:55 min

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August 04, 2022

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Simone Wanderoy*^1,2, Alina Rühmkorf*^1,2, Angelika B. Harbauer^3,4

¹TUM Medical Graduate Center,Technical University of Munich, ²Max Planck Institute of Neurobiology, ³TUM School of Medicine, Institute of Neuronal Cell Biology,Technical University of Munich, ⁴Munich Cluster for Systems Neurology

Capítulos

00:04Introduction
00:52Assembly of the Microfluidic Device
01:46Seeding and Maintaining of Neurons
03:13Staining with Mitochondrial Membrane Potential Sensitive Dye
04:14Live‐Cell Imaging
05:33Results: Mitochondrial Staining in Primary Hippocampal Neurons
06:10Conclusion

Summary

Tadução automática

本协议描述了微流体室中神经元线粒体的播种和染色。这些腔室中的流体压力梯度允许选择性处理轴突中的线粒体，以分析其性质以应对药理学挑战而不影响细胞体区室。

Tags

Microfluidics Axonal Mitochondria Neurodegenerative Diseases Fluidic Pressure Gradient Membrane Potential Hippocampal Neurons Cell Culture B-27 Neurobasal Media Microgrooves

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