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Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells
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Pesquisa do Câncer
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JoVE Journal Pesquisa do Câncer
Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells

Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells

DOI:
10.3791/64199-v

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06:29 min

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April 07, 2023

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, , , , ,
1Sylvester Comprehensive Cancer Center,University of Miami Miller School of Medicine

Capítulos

  • 00:04Introduction
  • 01:11Separation of Nuclear and Cytoplasmic Fractions
  • 01:56Validation of Nuclear-Cytoplasmic Separation
  • 04:35Results: Confirmation of RNA Purity of Cytoplasmic and Nuclear Fraction
  • 05:48Conclusion

Summary

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The present protocol offers an efficient and flexible method to isolate RNA from nuclear and cytoplasmic fractions using cultured cells, and then validate using qPCR. This effectively serves as a replacement for other RNA preparation kits.

Tags

Cytoplasmic RNANuclear RNACell FractionationLysis BufferQPCRMALAT1TUG1Cell CultureIntracellular InteractionsNuclear-cytoplasmic ExchangeCancer Development

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