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Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

DOI:
10.3791/64205-v

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10:09 min

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September 16, 2022

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, , , , , , ,
1Department of Anatomy and Molecular Cell Biology,Nagoya University Graduate School of Medicine, 2Division of Multicellular Circuit Dynamics, National Institute for Physiological Sciences,National Institutes of Natural Sciences, 3Department of System Science,Kobe University Graduate School of System Informatics, 4Department of Biology, Graduate School of Sciences,Kobe University, 5Department of Information Science,Kobe University Graduate School of System Informatics, 6Center of Optical Scattering Image Science,Kobe University

Capítulos

  • 00:04Introduction
  • 00:55Surgery and Adeno‐Associated Virus (AAV) Injection
  • 02:50Preparation for the Holographic Stimulation or Illumination System
  • 04:54Ca2+ Imaging Using an Image Sensor with Holographic Illumination
  • 06:06Two‐Photon Imaging with Optogenetics Using a Holographic Microscope
  • 08:04Results: Imaging and Functional Connectivity Using a Holographic Microscope
  • 09:26Conclusion

Summary

Tadução automática

Tadução automática

We developed a two-photon holographic microscope that can visualize, assess, and manipulate neural activity using high spatiotemporal resolution, with the aim of elucidating the pathogenesis of neuropsychiatric disorders that are associated with abnormal neural activity.

Tags

Two-photon Holographic MicroscopyNeuroactivityNeural Activity ManipulationSpatial Temporal ResolutionNeuropsychiatric DisordersAAV InjectionAgarose Brain WindowHolographic Stimulation System

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