Journal
/
Biologia
/
CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

DOI:
10.3791/64209-v

•

06:52 min

•

September 13, 2022

•

, , , , , , , , , ,
1Department of Biomedical Physiology and Kinesiology,Simon Fraser University

Capítulos

  • 00:05Introduction
  • 00:42Design of CRISPR Components for Precise Edits
  • 02:56Reporter Gene Screening of CRISPR-Cas9-Edited Larval Zebrafish
  • 03:42Genotyping of CRISPR-Cas9-Edited Larval Zebrafish
  • 05:20Results: Validation of Gene-Edits and Analysis of Cardiac Consequences
  • 06:13Conclusion

Summary

Tadução automática

Tadução automática

This protocol describes an approach to facilitate precise knock-in edits in zebrafish embryos using CRISPR-Cas9 technology. A phenotyping pipeline is presented to demonstrate the applicability of these techniques to model a Long QT Syndrome-associated gene variant.

Tags

CRISPR-Cas9Knock-inZebrafishLong QT SyndromeSgRNA DesignHDR TemplateSanger SequencingPhenotyping

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/64209?article_type=v

Optical Mapping of Langendorff-perfused Rat Hearts
check_url/pt/64209?article_type=v

In Situ Hybridization for the Precise Localization of Transcripts in Plants
check_url/pt/64209?article_type=v

In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina
check_url/pt/64209?article_type=v

In vivo Electroporation of Morpholinos into the Regenerating Adult Zebrafish Tail Fin
check_url/pt/64209?article_type=v

Gavaging Adult Zebrafish
check_url/pt/64209?article_type=v

Cell Labeling and Injection in Developing Embryonic Mouse Hearts
check_url/pt/64209?article_type=v

Recovery of Adult Zebrafish Hearts for High-throughput Applications
check_url/pt/64209?article_type=v

Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury
check_url/pt/64209?article_type=v

Local Field Fluorescence Microscopy: Imaging Cellular Signals in Intact Hearts
check_url/pt/64209?article_type=v

Monitoring On-Target Signaling Responses in Larval Zebrafish – Z-REX Unmasks Precise Mechanisms of Electrophilic Drugs and Metabolites

Read Article