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在根尖肠模型中使用路西法黄测定肠通透性
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model
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在根尖肠模型中使用路西法黄测定肠通透性

DOI:
10.3791/64215-v

•

09:48 min

•

July 27, 2022

•

, , , , , ,
1Division of Pediatric Surgery,Oklahoma Children’s Hospital, 2Oklahoma University Health Sciences Center, 3Department of Surgery,University of Oklahoma Health Sciences Center

Capítulos

  • 00:04Introduction
  • 00:59Generation of Apical‐Out (AO) Enteroids
  • 03:08Induction of Experimental Necrotizing Enterocolitis (NEC)
  • 05:06Measurement of Paracellular Permeability Utilizing Lucifer Yellow (LY)
  • 07:49Results: Determining the Intestinal Permeability Using Lucifer Yellow in an Apical‐Out Enteroid Model
  • 09:07Conclusion

Summary

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Tadução automática

本协议概述了一种在顶端肠模型中利用荧光花黄来确定肠道通透性的方法。该方法可用于确定模拟炎症性肠病（如坏死性小肠结肠炎）的肠样肠的细胞旁通透性。

Tags

Lucifer YellowApical-out Enteroid ModelIntestinal PermeabilityParacellular PathwaysNecrotizing EnterocolitisCell Recovery SolutionCentrifugation3D Model24-well PlateAO Enteroids

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