Journal
/
Medicina
/
In Vivo Luminal Measurement of Distension-Evoked Urothelial ATP Release in Rodents
JoVE Journal
Medicina
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Medicina
In Vivo Luminal Measurement of Distension-Evoked Urothelial ATP Release in Rodents

In Vivo Luminal Measurement of Distension-Evoked Urothelial ATP Release in Rodents

DOI:
10.3791/64227-v

•

09:17 min

•

September 07, 2022

•

, ,
1Department of Pharmacology and Chemical Biology,University of Pittsburgh School of Medicine

Capítulos

  • 00:05Introduction
  • 00:35Anesthesia and Ganglionic Block
  • 01:29Surgical Procedure: Suprapubic Bladder Catheterization
  • 02:29Transurethral Catheterization
  • 03:43Experimental Setup
  • 05:08Collection of Samples
  • 06:20Quantifying ATP from Collected Samples
  • 07:03Results: Measurement of Urothelial ATP Release In Vivo from the Lumen of the Bladder
  • 08:39Conclusion

Summary

Tadução automática

Tadução automática

This protocol describes the procedure for measuring ATP concentrations in the lumen of the bladder in an anesthetized rodent.

Tags

Artificial IntelligenceAI Writing AssistantsContent GenerationCopywritingText GenerationAI-powered Writing

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

The Use of Cystometry in Small Rodents: A Study of Bladder Chemosensation

In vivo Measurement of the Mouse Pulmonary Endothelial Surface Layer

Retinal Detachment Model in Rodents by Subretinal Injection of Sodium Hyaluronate

In vivo 19F MRI for Cell Tracking

Slow-release Drug Delivery through Elvax 40W to the Rat Retina: Implications for the Treatment of Chronic Conditions

Remote Limb Ischemic Preconditioning: A Neuroprotective Technique in Rodents

Intrauterine Telemetry to Measure Mouse Contractile Pressure In Vivo

Transcutaneous Assessment of Renal Function in Conscious Rodents

Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells

Preparation Steps for Measurement of Reactivity in Mouse Retinal Arterioles Ex Vivo

Read Article