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重组和表征具有可调电机驱动动力学和力学的肌动蛋白-微管复合材料

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Reconstituting and Characterizing Actin-Microtubule Composites with Tunable Motor-Driven Dynamics and Mechanics

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重组和表征具有可调电机驱动动力学和力学的肌动蛋白-微管复合材料

DOI:

10.3791/64228-v

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09:10 min

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August 25, 2022

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Mehrzad Sasanpour, Daisy H. Achiriloaie², Gloria Lee, Gregor Leech, Maya Hendija, K. Alice Lindsay, Jennifer L. Ross, Ryan J. McGorty, Rae M. Robertson-Anderson

¹Department of Physics and Biophysics,University of San Diego, ²W. M. Keck Science Department,Scripps College, Pitzer College, and Claremont McKenna College, ³Department of Physics,Syracuse University

Capítulos

00:05Introduction
01:01Preparation of Active Actin-Microtubule Composites Driven by Kinesin Motors
03:39Incorporating Passive Crosslinkers into Active Composites
05:11Results: Engineering and Characterizing the Tunable Dynamics and Structure of Motor-Driven Actin-Microtubule Composites
08:20Conclusion

Summary

Tadução automática

本文提出了用于工程和表征共纠缠肌动蛋白丝和微管的可调谐三维复合网络的协议。复合材料在肌球蛋白II和驱动蛋白马达的驱动下经历主动重组和弹道运动，并由肌动蛋白、微管、运动蛋白和被动交联剂的相对浓度进行调整。

Tags

Keywords: Actin Microtubules Cytoskeleton Reconstitution Composites Motor-driven Dynamics Mechanics Kinesin Myosin Active Matter Contraction Extension Coarsening Rupture Microscopy Confocal Imaging

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