É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:04Introduction
- 01:04Cell Lysis and Tn5 Tagmentation
- 01:52DNA Purification
- 02:57PCR Amplification
- 03:43Beads Purification
- 04:26Results: Comparison of TSS Enrichment Scores and Differential Peaks Obtained Using Optimized Cell‐Lysis Buffers, Cell Number Input, and Tn5 Concentration of ATAC‐Seq Libraries
- 06:33Conclusion
ATAC-seq is a DNA sequencing method that uses the hyperactive mutant transposase, Tn5, to map changes in chromatin accessibility mediated by transcription factors. ATAC-seq enables the discovery of the molecular mechanisms underlying phenotypic alterations in cancer cells. This protocol outlines optimization procedures for ATAC-seq in epithelial cell types, including cancer cells.
