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Primary Culture of Porcine Retinal Pigment Epithelial Cells

JoVE Journal
Biologia
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JoVE Journal Biologia

Primary Culture of Porcine Retinal Pigment Epithelial Cells

Primary Culture of Porcine Retinal Pigment Epithelial Cells

DOI:

10.3791/64244-v

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07:59 min

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September 23, 2022

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Feng Wen*^1,2,3, Yanzi Wang*^1,2,3, Danxue He^2,3, Chunyan Liao^2,3, Weijie Ouyang^2,3, Zuguo Liu^2,3, Wei Li^2,3, Yi Liao^2,3

¹Eye Institute of Xiamen University, Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, School of Medicine,Xiamen University, ²Department of Ophthalmology, Xiang’an Hospital of Xiamen University, School of Medicine,Xiamen University, ³Xiamen University Affiliated Xiamen Eye Center, School of Medicine,Xiamen University

Capítulos

00:04Introduction
01:15Preparation of Tissue Digestion Enzyme and Cell Culture Buffer
02:13Dissection of Porcine Eyeball RPE Cells
04:10Isolation and Culture of Porcine Eyeball RPE Cells
05:30Results: Cell Morphologies and Characterization of Cultured RPE Cells
07:27Conclusion

Summary

Tadução automática

Here, an easy-to-follow method to culture primary porcine retinal pigment epithelial cells in vitro is presented.

Tags

Keywords: Porcine Retinal Pigment Epithelial Cells RPE Disorders Primary RPE Cell Culture RPE Cell Model Physiological And Pathological Studies Laminin Cell Culture Protocol Eyeball Dissection

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