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Technical Applications of Microelectrode Array and Patch Clamp Recordings on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
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JoVE Journal Medicina
Technical Applications of Microelectrode Array and Patch Clamp Recordings on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Technical Applications of Microelectrode Array and Patch Clamp Recordings on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

DOI:
10.3791/64265-v

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10:30 min

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August 04, 2022

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, , , ,
1Stanford Cardiovascular Institute,Stanford University School of Medicine, 2Division of Cardiovascular Medicine, Department of Medicine,Stanford University School of Medicine, 3Department of Radiology,Stanford University School of Medicine

Capítulos

  • 00:04Introduction
  • 00:56Measurement of hiPSC‐CM Contraction Motion
  • 01:59Measurement of hiPSC‐CM Field Potential
  • 04:16Measurement of hiPSC‐CM Action Potential
  • 06:22Measurement of hiPSC‐CM Ca2+ Transient
  • 08:09Results: Contraction Motion, Microelectrode Array, Patch Clamp Recordings and Ca2+ Imaging on hiPSC‐CMs
  • 09:47Conclusion

Summary

Tadução automática

Tadução automática

Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have emerged as a promising in vitro model for drug-induced cardiotoxicity screening and disease modeling. Here, we detail a protocol for measuring the contractility and electrophysiology of hiPSC-CMs.

Tags

Keywords: Human IPSC-derived CardiomyocytesCardiac Safety AssessmentPre-clinical EvaluationMicroelectrode ArrayPatch ClampDisease ModelingDrug-induced Cardiotoxicity ScreeningPrecision MedicineCell CultureExtracellular MatrixCell SeedingSpontaneous Beating

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