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Biologia do Desenvolvimento
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Polysome Purification from Soybean Symbiotic Nodules
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Polysome Purification from Soybean Symbiotic Nodules

Polysome Purification from Soybean Symbiotic Nodules

DOI:
10.3791/64269-v

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07:02 min

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July 01, 2022

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, , , , , ,
1Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía,Universidad de la República, 2Departamento de Genómica,Instituto de Investigaciones Biológicas Clemente Estable, 3Department of Biology,University of Virginia, 4Departamento de Biología Celular y Molecular, Facultad de Ciencias,Universidad de la República

Capítulos

  • 00:05Introduction
  • 00:36Preparation of Cytosolic Extracts and Sucrose Cushions
  • 02:09Polysome Purification
  • 03:05RNA Extraction, Quality Control, and Precipitation
  • 05:24Results: Quality Assessment of the TOTAL and PAR Fractions
  • 06:24Conclusion

Summary

Tadução automática

Tadução automática

This protocol describes a method for eukaryotic polysome purification from intact soybean nodules. After sequencing, standard pipelines for gene expression analysis can be used to identify differentially expressed genes at the transcriptome and translatome levels.

Tags

Keywords: Polysome PurificationSoybeanSymbiotic NodulesCentrifugationRNA-SeqPolysome Extraction BufferSucrose CushionUltracentrifugationRNA Isolation

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