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Transplantation of Human Stem Cell-Derived GABAergic Neurons into the Early Postnatal Mouse Hippocampus to Mitigate Neurodevelopmental Disorders
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Transplantation of Human Stem Cell-Derived GABAergic Neurons into the Early Postnatal Mouse Hippocampus to Mitigate Neurodevelopmental Disorders

Transplantation of Human Stem Cell-Derived GABAergic Neurons into the Early Postnatal Mouse Hippocampus to Mitigate Neurodevelopmental Disorders

DOI:
10.3791/64272-v

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05:00 min

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November 11, 2022

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, , , , ,
1Cellular Neurophysiology and Epilepsy Group, Epilepsy Centre, Department of Clinical Sciences,Lund University Hospital, 2Experimental Epilepsy Group, Epilepsy Centre, Department of Clinical Sciences,Lund University Hospital, 3Molecular Neurophysiology and Epilepsy Group, Epilepsy Center, Department of Clinical Sciences,Lund University Hospital

Capítulos

  • 00:04Introduction
  • 00:50Preparation of the Single-Cell Suspension for Transplantation
  • 02:09Intrahippocampal Cell Transplantation
  • 03:24Results: hdINs into the Hippocampus of Newborn WT Mice and Cntnap2 KO Mice
  • 04:20Conclusion

Summary

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Transplantation of human pluripotent stem cell-derived GABAergic neurons generated by neuronal programming could be a potential treatment approach for neurodevelopmental disorders. This protocol describes the generation and transplantation of human stem cell-derived GABAergic neuronal precursors into the brains of neonatal mice, allowing long-term investigation of grafted neurons and evaluation of their therapeutic potential.

Tags

Human Stem CellsGABAergic NeuronsInterneuronsHippocampusNeurodevelopmental DisordersCell TransplantationCell TherapyTransgenic MiceSingle-cell SuspensionStereotaxic Injection

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