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将人干细胞衍生的GABA能神经元移植到出生后早期小鼠海马体中以减轻神经发育障碍

JoVE Journal
Neurociência

This content is Free Access.

JoVE Journal Neurociência

Transplantation of Human Stem Cell-Derived GABAergic Neurons into the Early Postnatal Mouse Hippocampus to Mitigate Neurodevelopmental Disorders

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将人干细胞衍生的GABA能神经元移植到出生后早期小鼠海马体中以减轻神经发育障碍

DOI:

10.3791/64272-v

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05:00 min

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November 11, 2022

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Ana Gonzalez-Ramos, Kerstin Laurin, Fredrik Berglind, Marco Ledri, Merab Kokaia, My Andersson

¹Cellular Neurophysiology and Epilepsy Group, Epilepsy Centre, Department of Clinical Sciences,Lund University Hospital, ²Experimental Epilepsy Group, Epilepsy Centre, Department of Clinical Sciences,Lund University Hospital, ³Molecular Neurophysiology and Epilepsy Group, Epilepsy Center, Department of Clinical Sciences,Lund University Hospital

Capítulos

00:04Introduction
00:50Preparation of the Single-Cell Suspension for Transplantation
02:09Intrahippocampal Cell Transplantation
03:24Results: hdINs into the Hippocampus of Newborn WT Mice and Cntnap2 KO Mice
04:20Conclusion

Summary

Tadução automática

移植由神经元编程产生的人类多能干细胞衍生的GABA能神经元可能是神经发育障碍的潜在治疗方法。该协议描述了人类干细胞来源的GABA能神经元前体的产生和移植到新生小鼠的大脑中，允许对移植神经元进行长期研究并评估其治疗潜力。

Tags

Human Stem Cells GABAergic Neurons Interneurons Hippocampus Neurodevelopmental Disorders Cell Transplantation Cell Therapy Transgenic Mice Single-cell Suspension Stereotaxic Injection

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