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小鼠胚胎中早期心脏祖细胞的实时成像

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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento

Live Imaging of Early Cardiac Progenitors in the Mouse Embryo

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小鼠胚胎中早期心脏祖细胞的实时成像

DOI:

10.3791/64273-v

•

07:02 min

•

July 12, 2022

•

Miquel Sendra, Jorge Mañes, Jorge N. Domínguez³, Miguel Torres

¹Cardiovascular Regeneration Program,Centro Nacional de Investigaciones Cardiovasculares (CNIC), ²Cardiovascular Development Group, Department of Experimental Biology,University of Jaén, Jaén, ³Fundación Medina, Granada

Capítulos

00:04Introduction
00:41Tools and Holder Customization
02:29Embryo Dissection
03:50Microscope Preparation, Embryo Mounting, and Image Acquisition
05:56Results: 3D Live Imaging of Cardiac Progenitor Cells in the Mouse Embryo
06:35Conclusion

Summary

Tadução automática

我们提出了小鼠胚胎培养和成像的详细方案，可实现心脏祖细胞的3D +时间成像。该视频工具包解决了成功进行实时成像所需的关键技能，否则很难从纯文本出版物中获得。

Tags

Live Imaging Early Cardiac Progenitors Mouse Embryo Cardiogenesis Embryo Development Tungsten Wire Glass Capillary Polymethyl Methacrylate Stereo Microscope Uterus Decidui Ectoplacental Cone Reichter’s Membrane Dissection Medium Culture Medium

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